Shimadzu Spd20a User Manual

| Symptom | Probable Cause | Corrective Action | |---------|----------------|--------------------| | High baseline noise | Air bubbles in cell | Flush with degassed mobile phase or remove cell and rinse. | | Negative peaks | Sample absorbs less than mobile phase at set wavelength | Change wavelength or check mobile phase UV cutoff. | | Lamp fails to ignite | Lamp expired or power supply issue | Check lamp hours; replace D2 lamp. | | No signal at high sensitivity | Flow cell window dirty | Clean cell with 1M HNO₃, then water, then methanol. | | Wavelength error | Stepper motor failure or filter stuck | Run Wavelength Calibration from service menu (qualified personnel only). |

The Nexera Virtual Advisor highlights key indicators often missed in the manual: Green: All systems go. Orange: The unit is in Sleep Mode to save lamp life. shimadzu spd20a user manual

Understanding the hardware capabilities is the first step toward optimal performance. 190 nm to 700 nm. Light Source: Deuterium (D2) lamp. Noise Level: 0.5 x 10⁻⁵ AU (world-class sensitivity). | Symptom | Probable Cause | Corrective Action